ABSTRACT
Objective To investigate the expression of interleukin-1 β(IL-1 β) and apoptosis in the hippocampus of rats with prolonged febrile seizures(PFS),and to provide the theoretical basis for exploring the brain damage of PFS and the effective prevention and control measures.Methods Ninety-six 14-day-old Sprague-Dawley rats were randomly divided into 3 groups:normal control group(NC group,n =32),hyperthermia group(HC group,n =32) and PFS group(n =32).Lipopolysaccharide combined low-dose Kainic acid of intraperitoneal injection were used to induce PFS.Then the histopathologic changes in hippocampus were viewed by HE staining and electronmicroscope,the expression of IL-1β protein in hippocampus of rats was detected with immunohistochemistry methods,the neuron apoptosis was observed by TdT-mediated dUTP nick end labeling,and the relationship of both was researched.Results 1.Thirty-two rats all appeared seizures in PFS group,the average rectal temperature was (39.3 ± 0.4) ℃ ; while the rats in other groups were not convulsions.2.The TUNEL positive cells in hippocampus CA1 of PFS group were more than that of NC group and HC group at 6 h after the PFS(all P ＜0.01),reached its highest level at 24 h.3.Measured by immunohistochemistry,the level of IL-1β in the hippocampus of PFS group was significantly higher than that of the NC group and HC group at 6 h after the PFS(all P ＜ 0.01).There was a positive correlation between the expression of IL-1 β and the apoptotic index in hippocampus(r =0.789,P ＜ 0.01).Conclusions IL-1 β expression in the hippocampus increases following PFS in rats,and the increased IL-1 β expression was correlated with neurocyte apoptosis.
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of apigenin on semen parameters in male mice.</p><p><b>METHODS</b>Totally 100 healthy male mice of Kunming strain were randomly divided into 5 groups according to the body weight: negative control, solvent control, low-dose apigenin, median-dose apigenin and high-dose apigenin, the latter three groups given intragastric apigenin at a fixed time every day for 7 and 14 days. At 35 days after the first medication, all the mice were killed and detected for the sperm motion parameters by computer aided sperm analysis (CASA).</p><p><b>RESULTS</b>There were no statistically significant differences in sperm motion parameters, density and motility between the negative control and the three apigenin groups after 7-day medication. At 14 days, the high-dose apigenin group showed remarkable decreases in average path velocity (VAP), straight line velocity (VSL), straightness (STR), wobbliness (WOB), the percentage of grade b sperm and sperm motility, and a significant increase in beat cross frequency (BCF) as compared with the negative control group (P < 0.05).</p><p><b>CONCLUSION</b>Apigenin affects sperm motility in male mice to a certain extent.</p>
Subject(s)
Animals , Male , Mice , Apigenin , Pharmacology , Mice, Inbred Strains , Semen , Sperm MotilityABSTRACT
<p><b>OBJECTIVE</b>To elucidate the molecular mechanism of X-linked adrenoleukodystrophy(ALD) in Chinese.</p><p><b>METHODS</b>Polymerase chain reaction in exon 1, exon 5 and their flanking sequences and direct DNA sequencing of ALD gene were performed in four patients, their mothers and twenty normal individuals as controls.</p><p><b>RESULTS</b>A splice mutation was identified in the interface of exon 5 and intron 5 (1875 G-->A). This splice mutation in 5' end of intron 5 might lead to abnormal splice in exon 5 and exon 6 and bring about unstable and abnormal ALD protein; the lignoceryl CoA ligase could not transport very long chain fatty acids (VLCFA) into peroxisome and could not function normally; consequently, defective beta-oxidation of VLCFA in peroxisome could result in an accumulation of VLCFAS in the central nervous system, adrenal gland and blood.</p><p><b>CONCLUSION</b>The splice mutation in 5' end of intron 5 leading to abnormal splice in exon 5 and exon 6 appears to be one of the causes of X-linked recessive adrenoleukodystrophy.</p>